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easygo! mouse il-18 one-step elisa kit  (Multi Sciences (Lianke) Biotech Co Ltd)


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    Multi Sciences (Lianke) Biotech Co Ltd easygo! mouse il-18 one-step elisa kit
    Easygo! Mouse Il 18 One Step Elisa Kit, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+il+18+elisa+kit/EasyGo!+Mouse+IL-18+One-Step+ELISA+Kit/custom%40ek218ega%4041807815
    Average 94 stars, based on 35 article reviews
    easygo! mouse il-18 one-step elisa kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Topical adhesive spatio-temporal nanosystem co-delivering chlorin e6 and HMGB1 inhibitor glycyrrhizic acid for in situ psoriasis chemo-phototherapy
    Article Snippet: Imiquimod ointment (5%) was purchased from Zhuhai United Laboratories Co., Ltd. China Mouse HMGB1 ELISA Kit was obtained from Elabscience® Biotechnology Co., Ltd. (Wuhan China). .. Mouse IL-18 ELISA Kit was obtained from Hangzhou Lianke Biotechnology Co., Ltd. (Hangzhou, China). .. Dihydroethidium (DHE) was obtained from Uelandy Biotechnology Co., Ltd. (US EVERBRIGHT, Suzhou, China).

    Article Title: Resistance to silicosis progression in mice with Ch25h downregulation: The involvement of NLRP3 inflammasome.
    Article Snippet: Silicosis is a fatal occupational lung disease characterized by persistent inflammation and irreversible fibrosis.. However, the pathogenesis of silicosis is currently unclear.. In this study, a mouse model of silicosis was established by intranasal instillation of silica, and transcriptomic alterations in lung tissues were assessed by mRNAsequencing.

    Article Title: Circulating Ly6C high monocyte-derived S100A4 + macrophages exacerbate neuroinflammation and impede recovery of traumatic brain injury.
    Article Snippet: Please cite this article as: Lu, Z., Wang, C., Zhu, Y., Zhao, J., Gu, Y., Zhu, X., Liao, W., Zhu, J., Jiang, R., Feng, S., Chen, T., Zhao, X., Wang, Z., Liu, Q., Gong, P., Yang, Y., Circulating Ly6Chigh monocyte-derived S100A4+ macrophages exacerbate neuroinflammation and impede recovery of traumatic brain injury, Brain, Behavior, and Immunity (2026), doi: https://doi.org/10.1016/j.bbi.2026.106472

    Article Title: SARM regulates cell apoptosis and inflammation during Toxoplasma gondii infection through a multistep mechanism.
    Article Snippet: .. To determine the effects of SARM on cytokine release, HT22 cells or BV2 cells that were overexpressing SARM were infected with T. gondii for 24 h. The levels of IL-18 secreted by the cells were quantified in 24 h supernatants by using mouse IL-18 ELISA kit (MultiSciences (Lianke) Biotech Co., Ltd., Hangzhou, China) according to the manufacturer’s instructions. .. All statistical analyses were done using GraphPad Prism 8.

    Article Title: Aerobic Exercise Training Exerts Neuroprotective Effects in Alzheimer's Disease Mice by Regulating Endoplasmic Reticulum Stress‐Autophagy Pathway‐Mediated Pyroptosis
    Article Snippet: .. Accordingly, IL‐1β and IL‐18 levels in the hippocampus were tested using the Mouse IL‐1β ELISA Kit (LIANKE BIOTECH, Hangzhou, Zhejiang, China, EK201B) and the Mouse IL‐18 ELISA Kit (LIANKE BIOTECH, EK218), respectively. ..

    Article Title: SARM regulates cell apoptosis and inflammation during Toxoplasma gondii infection through a multistep mechanism
    Article Snippet: .. To determine the effects of SARM on cytokine release, HT22 cells or BV2 cells that were overexpressing SARM were infected with T. gondii for 24 h. The levels of IL-18 secreted by the cells were quantified in 24 h supernatants by using mouse IL-18 ELISA kit (MultiSciences (Lianke) Biotech Co., Ltd., Hangzhou, China) according to the manufacturer’s instructions. .. All statistical analyses were done using GraphPad Prism 8.

    Article Title: Manganese composition for enhancing immunity
    Article Snippet: In short, the cDNA of the antigen fragment was inserted into the pET-21b vector (Novagen) and expressed in E. coli BL21 (DE3), and the recombinant protein was purified by Ni-NTA affinity column and then injected into mice or rabbits, thereby the antiserum that can recognize the corresponding antigen was obtained. .. Aluminum adjuvant (Imject Alum, Thermo, 77161), mouse IL-1beta ELISA Kit (MULTI SCIENCES, EK201B2/2), mouse IL-18 ELISA Kit (MULTI SCIENCES, EK2181), lipopolysaccharide (Sigma, L4130), ovalbumin (InvivoGen, #vac-pova) were all commercial products. .. Aluminum adjuvant (Imject Alum, Thermo, 77161), mouse IL-1beta ELISA Kit (MULTI SCIENCES, EK201B2/2), mouse IL-18 ELISA Kit (MULTI SCIENCES, EK2181), lipopolysaccharide (Sigma, L4130), ovalbumin (InvivoGen, #vac-pova) were all commercial products.

    Activity Assay:

    Article Title: Circulating Ly6C high monocyte-derived S100A4 + macrophages exacerbate neuroinflammation and impede recovery of traumatic brain injury.
    Article Snippet: Please cite this article as: Lu, Z., Wang, C., Zhu, Y., Zhao, J., Gu, Y., Zhu, X., Liao, W., Zhu, J., Jiang, R., Feng, S., Chen, T., Zhao, X., Wang, Z., Liu, Q., Gong, P., Yang, Y., Circulating Ly6Chigh monocyte-derived S100A4+ macrophages exacerbate neuroinflammation and impede recovery of traumatic brain injury, Brain, Behavior, and Immunity (2026), doi: https://doi.org/10.1016/j.bbi.2026.106472

    AST Assay:

    Article Title: Circulating Ly6C high monocyte-derived S100A4 + macrophages exacerbate neuroinflammation and impede recovery of traumatic brain injury.
    Article Snippet: Please cite this article as: Lu, Z., Wang, C., Zhu, Y., Zhao, J., Gu, Y., Zhu, X., Liao, W., Zhu, J., Jiang, R., Feng, S., Chen, T., Zhao, X., Wang, Z., Liu, Q., Gong, P., Yang, Y., Circulating Ly6Chigh monocyte-derived S100A4+ macrophages exacerbate neuroinflammation and impede recovery of traumatic brain injury, Brain, Behavior, and Immunity (2026), doi: https://doi.org/10.1016/j.bbi.2026.106472

    Infection:

    Article Title: SARM regulates cell apoptosis and inflammation during Toxoplasma gondii infection through a multistep mechanism.
    Article Snippet: .. To determine the effects of SARM on cytokine release, HT22 cells or BV2 cells that were overexpressing SARM were infected with T. gondii for 24 h. The levels of IL-18 secreted by the cells were quantified in 24 h supernatants by using mouse IL-18 ELISA kit (MultiSciences (Lianke) Biotech Co., Ltd., Hangzhou, China) according to the manufacturer’s instructions. .. All statistical analyses were done using GraphPad Prism 8.

    Article Title: SARM regulates cell apoptosis and inflammation during Toxoplasma gondii infection through a multistep mechanism
    Article Snippet: .. To determine the effects of SARM on cytokine release, HT22 cells or BV2 cells that were overexpressing SARM were infected with T. gondii for 24 h. The levels of IL-18 secreted by the cells were quantified in 24 h supernatants by using mouse IL-18 ELISA kit (MultiSciences (Lianke) Biotech Co., Ltd., Hangzhou, China) according to the manufacturer’s instructions. .. All statistical analyses were done using GraphPad Prism 8.

    Adjuvant:

    Article Title: Manganese composition for enhancing immunity
    Article Snippet: In short, the cDNA of the antigen fragment was inserted into the pET-21b vector (Novagen) and expressed in E. coli BL21 (DE3), and the recombinant protein was purified by Ni-NTA affinity column and then injected into mice or rabbits, thereby the antiserum that can recognize the corresponding antigen was obtained. .. Aluminum adjuvant (Imject Alum, Thermo, 77161), mouse IL-1beta ELISA Kit (MULTI SCIENCES, EK201B2/2), mouse IL-18 ELISA Kit (MULTI SCIENCES, EK2181), lipopolysaccharide (Sigma, L4130), ovalbumin (InvivoGen, #vac-pova) were all commercial products. .. Aluminum adjuvant (Imject Alum, Thermo, 77161), mouse IL-1beta ELISA Kit (MULTI SCIENCES, EK201B2/2), mouse IL-18 ELISA Kit (MULTI SCIENCES, EK2181), lipopolysaccharide (Sigma, L4130), ovalbumin (InvivoGen, #vac-pova) were all commercial products.



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    Activation of PDE4 induces neuronal pyroptosis 24 hours after SAH. (A) Representative TEM images of neurons. Red arrows: membrane pores. (B) Confocal IF staining of PDE4 (red) with neurons (NeuN, green) and proportion of PDE4-positive neurons. (C) Confocal IF staining of NLRP3 (red) with neurons (NeuN, green) and proportion of NLRP3-positive neurons. (D) Confocal IF staining of caspase-1 (red) with neurons (NeuN, green) and proportion of caspase-1-positive neurons. (E) Confocal IF staining of GSDMD (red) with neurons (NeuN, green) and proportion of GSDMD-positive neurons. Scale bars: 2 μm in A and 20 μm in B–E. (F) Representative western blot bands and relative protein expression of NLRP3, ASC, cleaved caspase-1, and GSDMD-N. (G) The levels of IL-1β <t>and</t> <t>IL-18</t> in the cerebral cortex of mice detected by enzyme-linked immunosorbent assay. Data are presented as mean ± SD; n = 6 per group. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance with Tukey’s post hoc test). ASC: Apoptosis-associated speck-like protein containing a caspase recruitment domain; Caspase-1: cysteinyl aspartate specific proteinase-1; ETZ: etazolate; GSDMD: gasderminD; IF: immunofluorescence; IL: interleukin; NLRP3: nucleotide-binding oligomerization domain-like receptor pyrin domain containing 3; PDE4: phosphodiesterase 4; SAH: subarachnoid hemorrhage; Sham: sham surgery; TEM: transmission electron microscopy.
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    Activation of PDE4 induces neuronal pyroptosis 24 hours after SAH. (A) Representative TEM images of neurons. Red arrows: membrane pores. (B) Confocal IF staining of PDE4 (red) with neurons (NeuN, green) and proportion of PDE4-positive neurons. (C) Confocal IF staining of NLRP3 (red) with neurons (NeuN, green) and proportion of NLRP3-positive neurons. (D) Confocal IF staining of caspase-1 (red) with neurons (NeuN, green) and proportion of caspase-1-positive neurons. (E) Confocal IF staining of GSDMD (red) with neurons (NeuN, green) and proportion of GSDMD-positive neurons. Scale bars: 2 μm in A and 20 μm in B–E. (F) Representative western blot bands and relative protein expression of NLRP3, ASC, cleaved caspase-1, and GSDMD-N. (G) The levels of IL-1β <t>and</t> <t>IL-18</t> in the cerebral cortex of mice detected by enzyme-linked immunosorbent assay. Data are presented as mean ± SD; n = 6 per group. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance with Tukey’s post hoc test). ASC: Apoptosis-associated speck-like protein containing a caspase recruitment domain; Caspase-1: cysteinyl aspartate specific proteinase-1; ETZ: etazolate; GSDMD: gasderminD; IF: immunofluorescence; IL: interleukin; NLRP3: nucleotide-binding oligomerization domain-like receptor pyrin domain containing 3; PDE4: phosphodiesterase 4; SAH: subarachnoid hemorrhage; Sham: sham surgery; TEM: transmission electron microscopy.
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    Image Search Results


    Activation of PDE4 induces neuronal pyroptosis 24 hours after SAH. (A) Representative TEM images of neurons. Red arrows: membrane pores. (B) Confocal IF staining of PDE4 (red) with neurons (NeuN, green) and proportion of PDE4-positive neurons. (C) Confocal IF staining of NLRP3 (red) with neurons (NeuN, green) and proportion of NLRP3-positive neurons. (D) Confocal IF staining of caspase-1 (red) with neurons (NeuN, green) and proportion of caspase-1-positive neurons. (E) Confocal IF staining of GSDMD (red) with neurons (NeuN, green) and proportion of GSDMD-positive neurons. Scale bars: 2 μm in A and 20 μm in B–E. (F) Representative western blot bands and relative protein expression of NLRP3, ASC, cleaved caspase-1, and GSDMD-N. (G) The levels of IL-1β and IL-18 in the cerebral cortex of mice detected by enzyme-linked immunosorbent assay. Data are presented as mean ± SD; n = 6 per group. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance with Tukey’s post hoc test). ASC: Apoptosis-associated speck-like protein containing a caspase recruitment domain; Caspase-1: cysteinyl aspartate specific proteinase-1; ETZ: etazolate; GSDMD: gasderminD; IF: immunofluorescence; IL: interleukin; NLRP3: nucleotide-binding oligomerization domain-like receptor pyrin domain containing 3; PDE4: phosphodiesterase 4; SAH: subarachnoid hemorrhage; Sham: sham surgery; TEM: transmission electron microscopy.

    Journal: Neural Regeneration Research

    Article Title: Phosphodiesterase 4 regulates pyroptosis in subarachnoid hemorrhage

    doi: 10.4103/NRR.NRR-D-24-01381

    Figure Lengend Snippet: Activation of PDE4 induces neuronal pyroptosis 24 hours after SAH. (A) Representative TEM images of neurons. Red arrows: membrane pores. (B) Confocal IF staining of PDE4 (red) with neurons (NeuN, green) and proportion of PDE4-positive neurons. (C) Confocal IF staining of NLRP3 (red) with neurons (NeuN, green) and proportion of NLRP3-positive neurons. (D) Confocal IF staining of caspase-1 (red) with neurons (NeuN, green) and proportion of caspase-1-positive neurons. (E) Confocal IF staining of GSDMD (red) with neurons (NeuN, green) and proportion of GSDMD-positive neurons. Scale bars: 2 μm in A and 20 μm in B–E. (F) Representative western blot bands and relative protein expression of NLRP3, ASC, cleaved caspase-1, and GSDMD-N. (G) The levels of IL-1β and IL-18 in the cerebral cortex of mice detected by enzyme-linked immunosorbent assay. Data are presented as mean ± SD; n = 6 per group. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance with Tukey’s post hoc test). ASC: Apoptosis-associated speck-like protein containing a caspase recruitment domain; Caspase-1: cysteinyl aspartate specific proteinase-1; ETZ: etazolate; GSDMD: gasderminD; IF: immunofluorescence; IL: interleukin; NLRP3: nucleotide-binding oligomerization domain-like receptor pyrin domain containing 3; PDE4: phosphodiesterase 4; SAH: subarachnoid hemorrhage; Sham: sham surgery; TEM: transmission electron microscopy.

    Article Snippet: The inflammatory factors of the brain cortex and primary neurons were measured using a mouse interleukin (IL)-1β ELISA kit (RK04878, Abclonal, Wuhan, China) and a mouse IL-18 ELISA kit (RK00104, Abclonal) following the manufacturer’s instructions.

    Techniques: Activation Assay, Membrane, Staining, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Binding Assay, Transmission Assay, Electron Microscopy

    Activation of PDE4 induces pyroptosis of primary neurons 24 hours after OxyHb induction. (A) Confocal IF staining of PDE4 (red) with primary neurons (MAP2, green) and PDE4 relative fluorescence intensity. (B) Confocal IF staining of NLRP3 (red) with primary neurons (MAP2, green) and NLRP3 relative fluorescence intensity. (C) Confocal IF staining of caspase-1 (red) with primary neurons (MAP2, green) and caspase-1 relative fluorescence intensity. (D) Confocal IF staining of GSDMD (red) with primary neurons (MAP2, green) and GSDMD relative fluorescence intensity. Scale bars: 20 μm in A–D. (E) Representative western blot bands and relative protein expression of PDE4, NLRP3, ASC, cleaved caspase-1, and GSDMD-N. (F) The levels of IL-1β and IL-18 in primary neurons detected by enzyme-linked immunosorbent assay. Data are presented as mean ± SD; n = 6 per group. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance with Tukey’s post hoc test or the Brown–Forsythe test). ASC: Apoptosis-associated speck-like protein containing a caspase recruitment domain; Caspase-1: cysteinyl aspartate specific proteinase-1; cells: primary neurons; ETZ: etazolate; GSDMD: gasderminD; IF: immunofluorescence; IL: interleukin; NLRP3: nucleotide-binding oligomerization domain-like receptor pyrin domain containing 3; OxyHb: oxyhemoglobin; PDE4: phosphodiesterase 4.

    Journal: Neural Regeneration Research

    Article Title: Phosphodiesterase 4 regulates pyroptosis in subarachnoid hemorrhage

    doi: 10.4103/NRR.NRR-D-24-01381

    Figure Lengend Snippet: Activation of PDE4 induces pyroptosis of primary neurons 24 hours after OxyHb induction. (A) Confocal IF staining of PDE4 (red) with primary neurons (MAP2, green) and PDE4 relative fluorescence intensity. (B) Confocal IF staining of NLRP3 (red) with primary neurons (MAP2, green) and NLRP3 relative fluorescence intensity. (C) Confocal IF staining of caspase-1 (red) with primary neurons (MAP2, green) and caspase-1 relative fluorescence intensity. (D) Confocal IF staining of GSDMD (red) with primary neurons (MAP2, green) and GSDMD relative fluorescence intensity. Scale bars: 20 μm in A–D. (E) Representative western blot bands and relative protein expression of PDE4, NLRP3, ASC, cleaved caspase-1, and GSDMD-N. (F) The levels of IL-1β and IL-18 in primary neurons detected by enzyme-linked immunosorbent assay. Data are presented as mean ± SD; n = 6 per group. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance with Tukey’s post hoc test or the Brown–Forsythe test). ASC: Apoptosis-associated speck-like protein containing a caspase recruitment domain; Caspase-1: cysteinyl aspartate specific proteinase-1; cells: primary neurons; ETZ: etazolate; GSDMD: gasderminD; IF: immunofluorescence; IL: interleukin; NLRP3: nucleotide-binding oligomerization domain-like receptor pyrin domain containing 3; OxyHb: oxyhemoglobin; PDE4: phosphodiesterase 4.

    Article Snippet: The inflammatory factors of the brain cortex and primary neurons were measured using a mouse interleukin (IL)-1β ELISA kit (RK04878, Abclonal, Wuhan, China) and a mouse IL-18 ELISA kit (RK00104, Abclonal) following the manufacturer’s instructions.

    Techniques: Activation Assay, Staining, Fluorescence, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Binding Assay

    Knockdown of endogenous PDE4 improved EBI and neuronal pyroptosis 24 hours after SAH. (A) Confocal IF staining of Cy3-conjugated PDE4 siRNA (red) with neurons (NeuN, green) and proportion of PDE4 siRNA-positive neurons. Scale bars: 50 μm. (B) The modified Garcia score, balance beam test, Rotarod test, and brain water content of all groups. (C) Representative western blot bands and relative protein expression of PDE4, NLRP3, ASC, cleaved caspase-1, and GSDMD-N. (D) The levels of IL-1β and IL-18 in the cerebral cortex of mice detected by enzyme-linked immunosorbent assay. Data are presented as mean ± SD; n = 6 per group; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance with Tukey’s post hoc test or the Brown–Forsythe test). ASC: Apoptosis-associated speck-like protein containing a caspase recruitment domain; Caspase-1: cysteinyl aspartate specific proteinase-1; EBI: early brain injury; GSDMD: gasderminD; IF: immunofluorescence; IL: interleukin; NLRP3: nucleotide-binding oligomerization domain-like receptor pyrin domain containing 3; PDE4: phosphodiesterase 4; SAH: subarachnoid hemorrhage; Scr: scrambled; Sham: sham surgery; siRNA: small interfering RNA.

    Journal: Neural Regeneration Research

    Article Title: Phosphodiesterase 4 regulates pyroptosis in subarachnoid hemorrhage

    doi: 10.4103/NRR.NRR-D-24-01381

    Figure Lengend Snippet: Knockdown of endogenous PDE4 improved EBI and neuronal pyroptosis 24 hours after SAH. (A) Confocal IF staining of Cy3-conjugated PDE4 siRNA (red) with neurons (NeuN, green) and proportion of PDE4 siRNA-positive neurons. Scale bars: 50 μm. (B) The modified Garcia score, balance beam test, Rotarod test, and brain water content of all groups. (C) Representative western blot bands and relative protein expression of PDE4, NLRP3, ASC, cleaved caspase-1, and GSDMD-N. (D) The levels of IL-1β and IL-18 in the cerebral cortex of mice detected by enzyme-linked immunosorbent assay. Data are presented as mean ± SD; n = 6 per group; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance with Tukey’s post hoc test or the Brown–Forsythe test). ASC: Apoptosis-associated speck-like protein containing a caspase recruitment domain; Caspase-1: cysteinyl aspartate specific proteinase-1; EBI: early brain injury; GSDMD: gasderminD; IF: immunofluorescence; IL: interleukin; NLRP3: nucleotide-binding oligomerization domain-like receptor pyrin domain containing 3; PDE4: phosphodiesterase 4; SAH: subarachnoid hemorrhage; Scr: scrambled; Sham: sham surgery; siRNA: small interfering RNA.

    Article Snippet: The inflammatory factors of the brain cortex and primary neurons were measured using a mouse interleukin (IL)-1β ELISA kit (RK04878, Abclonal, Wuhan, China) and a mouse IL-18 ELISA kit (RK00104, Abclonal) following the manufacturer’s instructions.

    Techniques: Knockdown, Staining, Modification, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Binding Assay, Small Interfering RNA

    Specific activation of NLRP3 reversed the neuroprotective effect of PDE4 inhibition 24 hours after SAH. (A) The modified Garcia score, balance beam test, Rotarod test, and brain water content of all groups. (B) Representative western blot bands and relative protein expression of PDE4, NLRP3, ASC, cleaved caspase-1, and GSDMD-N in the cerebral cortex of mice. (C) The levels of IL-1β and IL-18 in the cerebral cortex of mice detected by enzyme-linked immunosorbent assay. Data are presented as mean ± SD; n = 6 per group; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance with Tukey’s post hoc test or the Brown–Forsythe test). ASC: Apoptosis-associated speck-like protein containing a caspase recruitment domain; Caspase-1: cysteinyl aspartate specific proteinase-1; ETZ: etazolate; GSDMD: gasderminD; IL: interleukin; NIG: nigericin; NLRP3: nucleotide-binding oligomerization domain-like receptor pyrin domain containing 3; ns: not significant; PDE4: phosphodiesterase 4; SAH: subarachnoid hemorrhage; Sham: sham surgery.

    Journal: Neural Regeneration Research

    Article Title: Phosphodiesterase 4 regulates pyroptosis in subarachnoid hemorrhage

    doi: 10.4103/NRR.NRR-D-24-01381

    Figure Lengend Snippet: Specific activation of NLRP3 reversed the neuroprotective effect of PDE4 inhibition 24 hours after SAH. (A) The modified Garcia score, balance beam test, Rotarod test, and brain water content of all groups. (B) Representative western blot bands and relative protein expression of PDE4, NLRP3, ASC, cleaved caspase-1, and GSDMD-N in the cerebral cortex of mice. (C) The levels of IL-1β and IL-18 in the cerebral cortex of mice detected by enzyme-linked immunosorbent assay. Data are presented as mean ± SD; n = 6 per group; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 (one-way analysis of variance with Tukey’s post hoc test or the Brown–Forsythe test). ASC: Apoptosis-associated speck-like protein containing a caspase recruitment domain; Caspase-1: cysteinyl aspartate specific proteinase-1; ETZ: etazolate; GSDMD: gasderminD; IL: interleukin; NIG: nigericin; NLRP3: nucleotide-binding oligomerization domain-like receptor pyrin domain containing 3; ns: not significant; PDE4: phosphodiesterase 4; SAH: subarachnoid hemorrhage; Sham: sham surgery.

    Article Snippet: The inflammatory factors of the brain cortex and primary neurons were measured using a mouse interleukin (IL)-1β ELISA kit (RK04878, Abclonal, Wuhan, China) and a mouse IL-18 ELISA kit (RK00104, Abclonal) following the manufacturer’s instructions.

    Techniques: Activation Assay, Inhibition, Modification, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay, Binding Assay